Organization - Risø

Risø is a national laboratory under the Ministry of Science, Technology and Innovation.

It carries out research in science and technology, providing Danish society new opportunities for technological development.

The research creates new opportunities for Danish industry. It contributes to the development of environmentally acceptable methods for agricultural and industrial production as well as for the generation of the energy necessary for modern society.

Risø has a special responsibility for consolidatin the knowledge base on nuclear issues and providing consultancy to governmental authorities in this area.

Risø collaborates with universities, research institutes, technological institutes and the industrial sector on a national, European as well as on an international basis.

Risø places strong emphasis on the education of young researchers through PhD and postdoctorate programmes.

Research most relevant to BarleyGenomeNet is located in the Biosystems Department. The Biosystems Department is engaged in basic and applied research to improve the scientific basis for developing new methods and technology for the future, environmentally benign industrial and agricultural production, thus exerting less stress and strain on the environment.

Research relevant for BarleyGenomeNet

A method for single-cell transcriptome profiling in barley epidermis attacked by the powdery mildew fungus Blumeria graminis has been established. The contents of individual penetration-resistant cells mounting a successful papilla-response, infected cells enharbouring a fungal haustorium and uninoculated control cells, are collected in micro-capillaries in batches of 5 cells and delivered into extraction buffer within 90 s of commencing collection. The mRNA in the samples are then immediately captured on magnetic beads, purified and, after cDNA synthesis and PCR amplification, processed for the hybridisation experiments.

This technique allows for the first time to study host gene expression in this model pathosystem at single-cell resolution.

Barley Genome Net - Organisation - Risø

sequencer

backcross populations

breeding