Barley ESTs underlying GB and cMWG markers of this study were classified as showing homology or no homology to the rice genome (BLASTN E-value 1E-10 or > 1E-10, respectively). Based on their genetic postion they were grouped into bins of 20 cM (y-axis = number of ESTs, x-axis = genetic length given in cM). The distribution of ESTs without rice homology (upper panel of the histogram) was plotted against the distribution of ESTs showing homology to rice (lower panel of the histogram). The distribution of both classes of ESTs was following a very similar pattern along all chromosomes – no significantly different clustering to specific chromosomal regions could be observed.