This tool allows the identification and localization of perfect microsatellites as well as compound microsatellites which are interrupted by a certain number of bases.
In order to design primers flanking the
microsatellite loci, two perl scripts serve as interface modules for the
program-to-program data interchange between MISA and the primer modelling
software Primer3 (Whitehead Institute). For installation instructions of
Primer3 see http://www-genome.wi.mit.edu/genome_software/other/primer3.html.
Supplemental perl tools:
est_trimmer.pl - perl script useful for pre-preprocessing sequences
(invocation without parameters is explaining the syntax)
Primer 3 interface modules - perl scripts allowing the interaction
Questions and comments: Thomas Thiel
Last updated: 5/14/02