TABLE 1

Integration of 35 translocation breakpoints of chromosome 1H (5) into the Igri/Franka-derived RFLP linkage map

Genetic map

Physical map

   

TBb

Position (mGN)

Position (FL)

Markera

Position

(cM)

 

Obtained by

karyotyping

Refined by

PCR-mappingc

 

MWG2021.a

4.4

       

Ris45.b

8.8

       

MWG896

20.3

       
   

T54ai

S 38-50

S 38-50

S 0.88

   

T56ap

S 10-46

S 31-43

S 0.74

ABG500.a

30.3

       

MWG913

34.6

       
   

T57ac

S 24-50

S 31-43

S 0.74

MWG2056

36.0

       
   

T51ak

S 31-50

S 31-35

S 0.66

   

T57aj

S 19-40

S 23-40

S 0.63

   

T56x

S 10-32

S 23-32

S 0.55

   

T56ab

S 10-50

S 23-28

S 0.51

   

T51am

S 23-32

S 23-27

S 0.50

cMWG758 d

37.4

       
   

T57ab

S 23

S 23

S 0.46

   

T53ae

S 10-40

S 10-26

S 0.36

   

T57ad

S 11-17

S 11-17

S 0.28

   

T56aj

S 10-12

S 10-12

S 0.22

   

T54aq

S 10-11

S 10-11

S 0.21

   

T51a

0

0

0

CENTROMERE

(41.0)

 

(0)

   
   

T52ak

L 9-24

L 9-24

L 0.23

   

T53af

L 9-24

L 9-24

L 0.23

   

T56aa

L 9-24

L 9-24

L 0.23

   

T57ap

L 9-24

L 9-24

L 0.23

   

T51v

L 9-24

L 9-24

L 0.23

   

T52af

L 12-22

L 12-22

L 0.24

   

T54ah

L 11-24

L 11-24

L 0.24

   

T54e

L 9-24

L 15-24

L 0.27

   

T54ac

L 9-24

L 22-24

L 0.32

   

T54ap

L 13-24

L 22-24

L 0.32

   

T56ah

L 29-48

L 29-31

L 0.42

   

T54ad

L 29-41

L 29-35

L 0.44

   

T54af

L 29-62

L 29-35

L 0.44

   

T57ao

L 29-62

L 29-35

L 0.44

MWG800 d

44.6

       
   

T57ag

L 33-49

L 33-35

L 0.47

MWG506 d

50.5

       

Ris13 d

50.5

       

MWG2217

61.0

       
   

T56z

L 29-59

L 44-59

L 0.72

MWG943

61.0

       

MWG2077

63.9

       
   

T52ad

L 42-72

L 53-62

L 0.80

cMWG649b

80.3

       
   

T57af

L 29-62

L 53-62

L 0.80

cMWG706a

86.6

       

cMWG676

86.6

       

MWG733

101.0

       

MWG954

102.4

       

MWG984.b

108.2

       
   

T52as

L 46-72

L 57-66

L 0.86

MWG2028

111.2

       

cMWG701b

112.7

       
   

T52ah

L 66-72

L 66-67

L 0.92

MWG632.b

118.3

       

MWG912

129.7

       
   

T57an

L 29-72

L 66-67

L 0.92

ABG387a

132.6

       

a Markers representing reference points to the NABGMP Steptoe/Morex map (Graner et al. 1993) are shown in bold. Single-copy markers according to Andreas Graner (personal communication) are underlined

b Translocation breakpoints (TBs) used for defining chromosomal subregions for comparison of the physical and genetic maps are marked in bold

c The refined TB positions are based on a final analysis considering 240 genetically mapped TBs of 120 T-lines

d Arm-specificity of STS derivatives confirmed by PCR with DNA of microisolated telosomes of barley telotrisomic stocks (Singh and Tsuchiya 1982)

e Arm-specificity of STS derivatives confirmed by PCR with DNA of barley-wheat telosome addition lines (Islam et al. 1983), based on amplified polymorphic fragments between wheat (cv. Chinese Spring) and barley (cv. Betzes)